Workshop on Mammalian Expression Technologies
Oxford, 3-8 April 2011
Organisers: Yvonne Jones, Radu Aricescu, Susan Daenke, Christian Siebold, Karl Harlos, Margaret Jones, Benjamin Bishop, Amber Clayton, Veronica Chang, Weixian Lu, Tom Walter and Yuguang Zhao (all Oxford University, UK)
The course on Mammalian Expression Technologies was designed primarily for structural biologists who have prokaryotic expression experience and would like to use the mammalian expression system for more challenging targets.
Students, postdocs and experienced scientists from 11 different countries (Germany, Denmark, Belgium, UK, France, Austria, Spain, Greece, Czech Republic, Sweden and Switzerland) participated in the successful event and were happy to learn about the transnational access offered by P-CUBE.
During the course, apart from the excellent lectures, all the participants received hands-on training in mammalian cell culture, small and large scale transient gene expression and protein purification. Most of the participants were able to bring their own plasmids for small scale expression, and all got their own results by Western Blot analysis. Four participants brought their own constructs for large scale protein production, and all of them purified their own proteins with good yields. Three participants carried out the crystal screen with their purified proteins with a total 39 plates. One of the proteins showed crystals immediately, and conditions were optimised for obtaining bigger crystals.
The course participants particularly appreciated the opportunity to bring their own samples, the hands-on experience in the lab, the excellent supervision by the instructors and the great atmosphere during the course including the social events.
1. Mammalian expression platform overview
2. Mammalian cell lines for protein expression and glycosylation control
3. Mammalian expression constructs design
4. Small scale expression screen with Western Blot
5. Large scale expression and protein purification
6. Protein crystallisation
7. Protein structure case studies
PROGRAM (click here to download):