4–9 June 2012, Grenoble, France
Sponsorship kindly provided by EMBO, P-CUBE and Instruct
This EMBO practical course aims to teach the strategic use of various biochemical and biophysical techniques to facilitate structural studies of macromolecular complexes. The primary focus is on how to produce, purify and characterize multi-subunit protein and protein/nucleic acid complexes for structural analysis.
Topics include:
- expression in bacteria, insect and mammalian cells;
- affinity methods to purify protein components or entire complexes;
- biochemical and biophysical characterization of complexes;
- bioinformatic analysis of interaction networks;
- structural analysis by crystallography, cryo-electron microscopy, NMR,
and small angle X-ray/neutron scattering.
Emphasis will be placed on:
- the latest advances in isolating complexes directly from endogenous sources and in reconstituting complexes from individually purified components;
- techniques for assessing sample quality (homogeneity, stoichiometry, monodispersity);
- combining structure determination methods to obtain a multi-resolution view of a given complex
The course is primarily aimed at Ph.D students and post-docs engaged in challenging projects concerning the structural biology of macromolecular complexes.
The deadline for applications is: 4th April, 2012
A maximum of 20 participants will be selected to attend the course.
(Successful applicants will be notified by 20th April, 2012)
Please click here to register.
Monday 11 June - Saturday 16 June 2012, EMBL Hamburg, Germany
The course will cover theoretical and experimental approaches to Small Angle X-ray Scattering (SAXS), Macromolecular Crystallography (MX) and Electron Microscopy (EM) offered at EMBL Hamburg and EMBL Heidelberg. Combined use of these structural techniques will be demonstrated and discussed.
The course is aimed at PhD students and postdocs who have at least a basic understanding of one (or more) of the techniques listed above, and are interested in learning more about hybrid structural biology approaches.
Please note that there will be an additional possibility for a combination of SAXS with sample optimization and crystallization experiments, supported by the EU program P-CUBE. The selected participants will be offered the opportunity to send their samples in advance to the EMBL Hamburg high-throughput crystallization platform. Depending on the crystallization results, P-CUBE may sponsor optimization of the sample conditions, use of the EMBL Sample Preparation and Characterization (SPC) facility and more extensive SAXS experiments during the course. Travel support may also be offered for some of the P-CUBE sponsored participants. (Contact E-mail:
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).
Registration is open until the 1st of April, 2012.
Please click here to register.
3–11 September 2012, Hamburg, Germany
This intensive practical course is dedicated to modern methods in sample preparation and characterization of proteins for structural studies. It covers cloning and overexpression in bacterial and eukaryotic systems, protein purification with and without tags, biophysical characterization (including SAXS) and crystallization.
Deadline for registration: 20 May 2012
Deadline for abstract submission: 1 August 2012
Please click here to register.
October 17-24, 2012, EMBL Hamburg, Germany
This EMBO Practical Course is aimed at young scientists working in the field of structural biology including biochemists, biophysicists, and those specializing in other structural techniques (macromolecular crystallography, electron microscopy, NMR) and in bioinformatics.
Small-angle scattering (SAS) of X-rays and neutrons (SAXS/SANS) yields low resolution models of biological macromolecules in solution and also allows one to rapidly analyze structural changes in response to variations in external conditions.
Registration is open until the 31st of July, 2012.
Please click here to register. |
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